GeneTex
United States (US)

STAT4 (aa 613-627) antibody, Internal

GTX88040_2677_WB_18122102_796.jpg

Cat No. GTX88040

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-0.5μg/ml
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88040-PEP).

Predict Reactivity

Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DHSESGEVRFHSVEP, from the internal region of the protein sequence according to NP_003142.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Signal Transducer And Activator Of Transcription 4,Sleb11,Stat4

Cellular Localization

Translocated into the nucleus in response to phosphorylation.

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES
GTX88040_2677_WB_18122102_796.jpg

GTX88040 WB Image

WB analysis of HEK293 overexpressing human STAT4 with C-terminal MYC tag using GTX88040 STAT4 (aa 613-627) antibody, Internal.
Lane A : primary antibody
Lane B : Mock-transfected HEK293 probed with primary antibody
Lane C : Tag antibody
Dilution : 0.5µg/ml
Loading : 10µg protein in RIPA buffer

Package List Price ($)
$ 329