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STAT4 antibody [GT1244]

Recombinant antibody
Anti-STAT4 antibody [GT1244] used in Western Blot (WB). GTX02841
Anti-STAT4 antibody [GT1244] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02841
Anti-STAT4 antibody [GT1244] used in Western Blot (WB). GTX02841
Anti-STAT4 antibody [GT1244] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02841

Cat. No. GTX02841

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1244

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all STAT4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human STAT4

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal transducer and activator of transcription 4 , SLEB11

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-STAT4 antibody [GT1244] used in Western Blot (WB). GTX02841

GTX02841 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with STAT4 antibody [GT1244] (GTX02841) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT4 antibody [GT1244] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02841

GTX02841 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX02841 STAT4 antibody [GT1244].
Dilution : 1:100
Blue : DAPI for nuclear staining.

Anti-STAT4 antibody [GT1244] used in Western Blot (WB). GTX02841

GTX02841 WB Image

WB analysis of various samples using GTX02841 STAT4 antibody [GT1244].
Dilution : 1:1000
Loading : 25μg

Anti-STAT4 antibody [GT1244] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02841

GTX02841 ICC/IF Image

ICC/IF analysis of C6 cells using GTX02841 STAT4 antibody [GT1244].
Dilution : 1:100
Blue : DAPI for nuclear staining.

REFERENCE

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REVIEW

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Package List Price ($)
$ 419