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STAT5A antibody [6D4]

Anti-STAT5A antibody [6D4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60652
Anti-STAT5A antibody [6D4] used in Flow cytometry (FACS). GTX60652
Anti-STAT5A antibody [6D4] used in IHC (Paraffin sections) (IHC-P). GTX60652
Anti-STAT5A antibody [6D4] used in IHC (Paraffin sections) (IHC-P). GTX60652
Anti-STAT5A antibody [6D4] used in ELISA (ELISA). GTX60652
Anti-STAT5A antibody [6D4] used in Western Blot (WB). GTX60652

Cat. No. GTX60652

Host

Mouse

Clonality

Monoclonal

Clone Name

6D4

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

Human
Package
100 μg ($409)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/50
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human STAT5A (AA: 583-794 ) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

signal transducer and activator of transcription 5A , MGF , STAT5

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-STAT5A antibody [6D4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60652

GTX60652 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60652 STAT5A antibody [6D4].
Green : STAT5A
Blue: DRAQ5 fluorescent DNA dye

Anti-STAT5A antibody [6D4] used in Flow cytometry (FACS). GTX60652

GTX60652 FACS Image

FACS analysis of K562 cells using GTX60652 STAT5A antibody [6D4].
Green : STAT5A
Red : negative control

Anti-STAT5A antibody [6D4] used in IHC (Paraffin sections) (IHC-P). GTX60652

GTX60652 IHC-P Image

IHC-P analysis of ovarian cancer tissue using GTX60652 STAT5A antibody [6D4].

Anti-STAT5A antibody [6D4] used in IHC (Paraffin sections) (IHC-P). GTX60652

GTX60652 IHC-P Image

IHC-P analysis of rectum cancer tissue using GTX60652 STAT5A antibody [6D4].

Anti-STAT5A antibody [6D4] used in ELISA (ELISA). GTX60652

GTX60652 ELISA Image

ELISA analysis of antigen using GTX60652 STAT5A antibody [6D4].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-STAT5A antibody [6D4] used in Western Blot (WB). GTX60652

GTX60652 WB Image

WB analysis of K562 (1), MOLT4 (2), HeLa (3), Jurkat (4), and A431 (5) cell lysate using GTX60652 STAT5A antibody [6D4].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409