APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/50 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant fragment of human STAT5A (AA: 583-794 ) expressed in E. Coli.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
signal transducer and activator of transcription 5A , MGF , STAT5
Cellular Localization
Cytoplasm,Nucleus
Background
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-STAT5A antibody [6D4] used in Flow cytometry (FACS). GTX60652](/templates/default/js/lightbox/images/loading.gif "Anti-STAT5A antibody [6D4] used in Flow cytometry (FACS). GTX60652")
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GTX60652 FACS Image
FACS analysis of K562 cells using GTX60652 STAT5A antibody [6D4].
Green : STAT5A
Red : negative control
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GTX60652 IHC-P Image
IHC-P analysis of rectum cancer tissue using GTX60652 STAT5A antibody [6D4].
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GTX60652 IHC-P Image
IHC-P analysis of ovarian cancer tissue using GTX60652 STAT5A antibody [6D4].
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GTX60652 WB Image
WB analysis of K562 (1), MOLT4 (2), HeLa (3), Jurkat (4), and A431 (5) cell lysate using GTX60652 STAT5A antibody [6D4].
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GTX60652 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX60652 STAT5A antibody [6D4].
Green : STAT5A
Blue: DRAQ5 fluorescent DNA dye
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GTX60652 ELISA Image
ELISA analysis of antigen using GTX60652 STAT5A antibody [6D4].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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REFERENCE
There are currently no references for STAT5A antibody [6D4] (GTX60652). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for STAT5A antibody [6D4] (GTX60652). Be the first to share your experience with this product.
![Anti-STAT5A antibody [6D4] used in Flow cytometry (FACS). GTX60652](/upload/website/prouct_img/small/GTX60652/GTX60652_20170912_FACS_w_23061123_622.webp "Anti-STAT5A antibody [6D4] used in Flow cytometry (FACS). GTX60652")
![Anti-STAT5A antibody [6D4] used in IHC (Paraffin sections) (IHC-P). GTX60652](/upload/website/prouct_img/small/GTX60652/GTX60652_20170912_IHC-P_1_w_23061123_987.webp "Anti-STAT5A antibody [6D4] used in IHC (Paraffin sections) (IHC-P). GTX60652")
![Anti-STAT5A antibody [6D4] used in IHC (Paraffin sections) (IHC-P). GTX60652](/upload/website/prouct_img/small/GTX60652/GTX60652_20170912_IHC-P_w_23061123_446.webp "Anti-STAT5A antibody [6D4] used in IHC (Paraffin sections) (IHC-P). GTX60652")
![Anti-STAT5A antibody [6D4] used in Western Blot (WB). GTX60652](/upload/website/prouct_img/small/GTX60652/GTX60652_20170912_WB_w_23061123_273.webp "Anti-STAT5A antibody [6D4] used in Western Blot (WB). GTX60652")
![Anti-STAT5A antibody [6D4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60652](/upload/website/prouct_img/small/GTX60652/GTX60652_20170912_ICCIF_w_23061123_457.webp "Anti-STAT5A antibody [6D4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60652")
![Anti-STAT5A antibody [6D4] used in ELISA (ELISA). GTX60652](/upload/website/prouct_img/small/GTX60652/GTX60652_20170912_ELISA_w_23061123_746.webp "Anti-STAT5A antibody [6D4] used in ELISA (ELISA). GTX60652")
![Anti-STAT5A antibody [6D4] used in Flow cytometry (FACS). GTX60652](/upload/website/prouct_img/normal/GTX60652/GTX60652_20170912_FACS_w_23061123_622.webp "Anti-STAT5A antibody [6D4] used in Flow cytometry (FACS). GTX60652")
![Anti-STAT5A antibody [6D4] used in IHC (Paraffin sections) (IHC-P). GTX60652](/upload/website/prouct_img/normal/GTX60652/GTX60652_20170912_IHC-P_1_w_23061123_987.webp "Anti-STAT5A antibody [6D4] used in IHC (Paraffin sections) (IHC-P). GTX60652")
![Anti-STAT5A antibody [6D4] used in IHC (Paraffin sections) (IHC-P). GTX60652](/upload/website/prouct_img/normal/GTX60652/GTX60652_20170912_IHC-P_w_23061123_446.webp "Anti-STAT5A antibody [6D4] used in IHC (Paraffin sections) (IHC-P). GTX60652")
![Anti-STAT5A antibody [6D4] used in Western Blot (WB). GTX60652](/upload/website/prouct_img/normal/GTX60652/GTX60652_20170912_WB_w_23061123_273.webp "Anti-STAT5A antibody [6D4] used in Western Blot (WB). GTX60652")
![Anti-STAT5A antibody [6D4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60652](/upload/website/prouct_img/normal/GTX60652/GTX60652_20170912_ICCIF_w_23061123_457.webp "Anti-STAT5A antibody [6D4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60652")
![Anti-STAT5A antibody [6D4] used in ELISA (ELISA). GTX60652](/upload/website/prouct_img/normal/GTX60652/GTX60652_20170912_ELISA_w_23061123_746.webp "Anti-STAT5A antibody [6D4] used in ELISA (ELISA). GTX60652")