GeneTex
United States (US)

STAT5A (phospho Ser780) antibody

GTX32268_20190131_ICCIF_19013101_924.jpg
GTX32268_20190131_WB_19013101_297.jpg
GTX32268_20190131_IHC-P_19013101_175.jpg

Cat No. GTX32268

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

91 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of STAT5A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Signal Transducer And Activator Of Transcription 5A,Mgf,Stat5,Stat5A

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]

Database

Research Area

DATA IMAGES
GTX32268_20190131_ICCIF_19013101_924.jpg

GTX32268 ICC/IF Image

ICC/IF analysis of formalin-fixed HepG2 cells using GTX32268 STAT5A (phospho Ser780) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX32268_20190131_WB_19013101_297.jpg

GTX32268 WB Image

WB analysis of EGF-treated A431 (A), HepG2 (B) whole cell lysates using GTX32268 STAT5A (phospho Ser780) antibody.

GTX32268_20190131_IHC-P_19013101_175.jpg

GTX32268 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32268 STAT5A (phospho Ser780) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)