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STAT5A (phospho Tyr694) antibody [5F6.F1]

Anti-STAT5A (phospho Tyr694) antibody [5F6.F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48647
Anti-STAT5A (phospho Tyr694) antibody [5F6.F1] used in IHC (Paraffin sections) (IHC-P). GTX48647
Anti-STAT5A (phospho Tyr694) antibody [5F6.F1] used in Western Blot (WB). GTX48647

Cat No. GTX48647

Host

Mouse

Clonality

Monoclonal

Clone Name

5F6.F1

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:1000
IHC-P 20 - 40 μg/ml
ELISA 1:20000
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Phospho STAT5 pY694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

signal transducer and activator of transcription 5A , AA959963 , STAT5

Cellular Localization

Cytoplasm,Nucleus

Background

Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5and Stat5. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus.

Database

Research Area

DATA IMAGES

Anti-STAT5A (phospho Tyr694) antibody [5F6.F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48647

GTX48647 ICC/IF Image

Anti-STAT5 pY694 (MOUSE) Monoclonal Antibody detecting STAT5 in 3T3 cells (immunofluorescent STED microscopy). Red represents STAT5 pY694 protein. Green represents tubulin.

Anti-STAT5A (phospho Tyr694) antibody [5F6.F1] used in IHC (Paraffin sections) (IHC-P). GTX48647

GTX48647 IHC-P Image

Immunohistochemistry using GeneTex Immunochemicals Anti-STAT5 pY694 monoclonal antibody shows detection of phosphorylated STAT5 pY694 in human breast tissue (40X). The antibody was used a dilution to 20 μg/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Anti-STAT5A (phospho Tyr694) antibody [5F6.F1] used in Western Blot (WB). GTX48647

GTX48647 WB Image

Western blot using GeneTex's Protein A purified Mouse Monoclonal anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution, overnight at 4º C. For detection DyLight800 conjugated Goat-anti-Mouse IgG was used at a 1:20,000 dilution for 30 min at room temperature.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf