APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human Phospho-Stat5 (Y694).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
MGF,STAT5,STAT5A,signal transducer and activator of transcription 5A
Cellular Localization
Cytoplasm,Nucleus
Background
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]
Database
Research Area
DATA IMAGES
![Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in Western Blot (WB). GTX00970](/templates/default/js/lightbox/images/loading.gif "Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in Western Blot (WB). GTX00970")
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GTX00970 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT5A (phospho Tyr694) antibody [GT1208] (GTX00970) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The observed M.W. is based on the publication: PMID: 16461551
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GTX00970 WB Image
WB analysis of TF-1 cell extracts with/without GM-CSF (25 ng/ml, 37ºC, 30 mins) treatment after serum-starvation overnight using GTX00970 STAT5A (phospho Tyr694) antibody [GT1208].
Dilution : 1:1000
Loading : 25 μg
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GTX00970 IHC-P Image
IHC-P analysis of rat brain tissue section using GTX00970 STAT5A (phospho Tyr694) antibody [GT1208].
Dilution : 1:100
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GTX00970 IHC-P Image
IHC-P analysis of human oophoroma tissue section using GTX00970 STAT5A (phospho Tyr694) antibody [GT1208].
Dilution : 1:100
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GTX00970 IHC-P Image
IHC-P analysis of mouse kidney tissue section using GTX00970 STAT5A (phospho Tyr694) antibody [GT1208].
Dilution : 1:100
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REFERENCE
There are currently no references for STAT5A (phospho Tyr694) antibody [GT1208] (GTX00970). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for STAT5A (phospho Tyr694) antibody [GT1208] (GTX00970). Be the first to share your experience with this product.
![Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in Western Blot (WB). GTX00970](/upload/website/prouct_img/small/GTX00970/GTX00970_4000000176_20200410_WB_treatment_hIFN-alpha_w_23053121_208.webp "Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in Western Blot (WB). GTX00970")
![Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in Western Blot (WB). GTX00970](/upload/website/prouct_img/small/GTX00970/GTX00970_20200508_WB_w_23053121_684.webp "Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in Western Blot (WB). GTX00970")
![Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in IHC (Paraffin sections) (IHC-P). GTX00970](/upload/website/prouct_img/small/GTX00970/GTX00970_20200508_IHC-P_2_w_23053121_933.webp "Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in IHC (Paraffin sections) (IHC-P). GTX00970")
![Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in IHC (Paraffin sections) (IHC-P). GTX00970](/upload/website/prouct_img/small/GTX00970/GTX00970_20200508_IHC-P_1_w_23053121_240.webp "Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in IHC (Paraffin sections) (IHC-P). GTX00970")
![Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in IHC (Paraffin sections) (IHC-P). GTX00970](/upload/website/prouct_img/small/GTX00970/GTX00970_20200508_IHC-P_w_23053121_753.webp "Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in IHC (Paraffin sections) (IHC-P). GTX00970")
![Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in Western Blot (WB). GTX00970](/upload/website/prouct_img/normal/GTX00970/GTX00970_4000000176_20200410_WB_treatment_hIFN-alpha_w_23053121_208.webp "Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in Western Blot (WB). GTX00970")
![Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in Western Blot (WB). GTX00970](/upload/website/prouct_img/normal/GTX00970/GTX00970_20200508_WB_w_23053121_684.webp "Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in Western Blot (WB). GTX00970")
![Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in IHC (Paraffin sections) (IHC-P). GTX00970](/upload/website/prouct_img/normal/GTX00970/GTX00970_20200508_IHC-P_2_w_23053121_933.webp "Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in IHC (Paraffin sections) (IHC-P). GTX00970")
![Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in IHC (Paraffin sections) (IHC-P). GTX00970](/upload/website/prouct_img/normal/GTX00970/GTX00970_20200508_IHC-P_1_w_23053121_240.webp "Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in IHC (Paraffin sections) (IHC-P). GTX00970")
![Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in IHC (Paraffin sections) (IHC-P). GTX00970](/upload/website/prouct_img/normal/GTX00970/GTX00970_20200508_IHC-P_w_23053121_753.webp "Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in IHC (Paraffin sections) (IHC-P). GTX00970")