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STAT5A (phospho Tyr694) antibody [GT1208]

Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in Western Blot (WB). GTX00970
Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in Western Blot (WB). GTX00970
Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in IHC (Paraffin sections) (IHC-P). GTX00970
Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in IHC (Paraffin sections) (IHC-P). GTX00970
Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in IHC (Paraffin sections) (IHC-P). GTX00970

Cat. No. GTX00970

Host

Rabbit

Clonality

Monoclonal

Clone Name

GT1208

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH7.3), 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Phospho-Stat5 (Y694).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MGF,STAT5,STAT5A,signal transducer and activator of transcription 5A

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]

Database

Research Area

DATA IMAGES

Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in Western Blot (WB). GTX00970

GTX00970 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT5A (phospho Tyr694) antibody [GT1208] (GTX00970) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The observed M.W. is based on the publication: PMID: 16461551

Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in Western Blot (WB). GTX00970

GTX00970 WB Image

WB analysis of TF-1 cell extracts with/without GM-CSF (25 ng/ml, 37ºC, 30 mins) treatment after serum-starvation overnight using GTX00970 STAT5A (phospho Tyr694) antibody [GT1208].
Dilution : 1:1000
Loading : 25 μg

Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in IHC (Paraffin sections) (IHC-P). GTX00970

GTX00970 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX00970 STAT5A (phospho Tyr694) antibody [GT1208].
Dilution : 1:100

Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in IHC (Paraffin sections) (IHC-P). GTX00970

GTX00970 IHC-P Image

IHC-P analysis of mouse kidney tissue section using GTX00970 STAT5A (phospho Tyr694) antibody [GT1208].
Dilution : 1:100

Anti-STAT5A (phospho Tyr694) antibody [GT1208] used in IHC (Paraffin sections) (IHC-P). GTX00970

GTX00970 IHC-P Image

IHC-P analysis of human oophoroma tissue section using GTX00970 STAT5A (phospho Tyr694) antibody [GT1208].
Dilution : 1:100

REFERENCE

There are currently no references for STAT5A (phospho Tyr694) antibody [GT1208] (GTX00970). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for STAT5A (phospho Tyr694) antibody [GT1208] (GTX00970). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399