*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
HeLa starvation 16 hr (100 ng/ml hIFN-alpha for 5 min)
Mouse, Rat, Zebrafish, Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
1XPBS (pH7), 1% BSA, 20% Glycerol
0.025% ProClin 300
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1.04 mg/ml (Please refer to the vial label for the specific concentration.)
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr694 of human STAT5A. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
signal transducer and activator of transcription 5A , MGF , STAT5
Cytoplasm , Nucleus
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq]
GTX133947 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT5A (phospho Tyr694) antibody (GTX133947) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.