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STAT5A (phospho Tyr694) antibody

Orthogonal
Anti-STAT5A (phospho Tyr694) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13593
Anti-STAT5A (phospho Tyr694) antibody used in IHC (Paraffin sections) (IHC-P). GTX13593
Anti-STAT5A (phospho Tyr694) antibody used in ChIP assay (ChIP assay). GTX13593
Anti-STAT5A (phospho Tyr694) antibody used in Western Blot (WB). GTX13593

Cat. No. GTX13593

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IP, ChIP assay

Reactivity

 Human, Mouse
Package
50 μl ($399)
See all STAT5A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:250
IHC-P 1:10-1:100
FACS 1:20
IP Assay dependent
ChIP assay 1-5 μg
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human STAT5a that contains tyrosine 694. The sequence is conserved in mouse and rat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_372365

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal transducer and activator of transcription 5A , MGF , STAT5

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]

Database

Research Area

DATA IMAGES

Anti-STAT5A (phospho Tyr694) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13593

GTX13593 ICC/IF Image

Orthogonal

ICC/IF analysis of HeLa cells treated with 5uM of TNF-alpha for 30 minutes using GTX13593 STAT5A (phospho Tyr694) antibody. Panel e is untreated cell with no signal. Panel f is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutesDilution: 1:250

Anti-STAT5A (phospho Tyr694) antibody used in IHC (Paraffin sections) (IHC-P). GTX13593

GTX13593 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX13593 STAT5A (phospho Tyr694) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-STAT5A (phospho Tyr694) antibody used in ChIP assay (ChIP assay). GTX13593

GTX13593 ChIP assay Image

ChIP analysis of 2 million HeLa cells treated with 100ng/ml of IFN gamma for 45 minutes using GTX13593 STAT5A (phospho Tyr694) antibody. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to the promoter of COX1, AP1 gene as positive control target and the inactive SAT2 used as negative control target.

Anti-STAT5A (phospho Tyr694) antibody used in Western Blot (WB). GTX13593

GTX13593 WB Image

Orthogonal

WB (peptide competition) analysis of 3T3-L1 cells treated with 20 ng/mL LIF for 10 minutes (Lane 2-5) using GTX13593 STAT5A (phospho Tyr694) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

REFERENCE

Application Reference

Lv H et al. Nat Commun 2024; 15 (1) : 6 TET2-mediated tumor cGAS triggers endothelial STING activation to regulate vasculature remodeling and anti-tumor immunity in liver cancer
Application : IP
Reactivity : Mouse
Chi M et al. Sci Rep 2016; 6 : 33132 Phosphorylation of calcium/calmodulin-stimulated protein kinase II at T286 enhances invasion and migration of human breast cancer cells.
Application : WB
Reactivity : Human
Lu JW et al. Cancer Lett 2016; (Epub) * is selectively cytotoxic in acute myeloid leukemia cells with flt3-internal tandem duplication (flt3-itd).
Application : WB
Reactivity : Human
Uchino Y et al. Cancer Cell Int 2015; 15 : 30 Granulocyte colony-stimulating factor potentiates all-trans retinoic acid-induced granulocytic differentiation in acute promyelocytic leukemia cell line HT93A.
Vazquez-Mellado MJ et al. Life Sci 2015; 137 (Epub) : 142-9 erythropoietin protects neuroblastoma cells against * and * by activating erk and akt pathways but has no effect in kidney cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399