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STAT5B antibody [GT1160]

Recombinant antibody KO/KD
Anti-STAT5B antibody [GT1160] used in Western Blot (WB). GTX08967
Anti-STAT5B antibody [GT1160] used in Western Blot (WB). GTX08967
Anti-STAT5B antibody [GT1160] used in Western Blot (WB). GTX08967

Cat. No. GTX08967

3

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1160

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all STAT5B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from hmuan STAT5b

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal transducer and activator of transcription 5B , STAT5

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-STAT5B antibody [GT1160] used in Western Blot (WB). GTX08967

GTX08967 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with STAT5B antibody [GT1160] (GTX08967) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT5B antibody [GT1160] used in Western Blot (WB). GTX08967

GTX08967 WB Image

WB analysis of various samples using GTX08967 STAT5B antibody [GT1160].
Dilution : 1:1000
Loading : 25μg

Anti-STAT5B antibody [GT1160] used in Western Blot (WB). GTX08967

GTX08967 WB Image

KO/KD

WB analysis of Wild-type (WT) and STAT5B knockout (KO) HeLa cell extracts using GTX08967 STAT5B antibody [GT1160].
Dilution : 1:1000
Loading : 25μg

REFERENCE

There are currently no references for STAT5B antibody [GT1160] (GTX08967). Be the first to share your publications with this product.
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REVIEW

STAT5B antibody [GT1160] Cat. No. GTX08967

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-STAT5B antibody [GT1160] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX08967
Date : Anonymous submitted on 09-Nov-2022
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HAP1 WT and STAT5B KO cells
Blocking : PBS with 5% BSA, 5% goat serum and 0.01% Triton X-100, RT°C, 30 minHr
Permeabilization /Antigen retrieval : PBS with 0,1% Triton X-100, RT°C, 10 minHr
Figure Note : WT cells: Green dashed line KO cells: Magenta dashed line Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-STAT5B antibody [GT1160] used in Immunoprecipitation (IP). GTX08967
Date : Anonymous submitted on 09-Nov-2022
Score :
Application Tested : IP
Sample Species : Hu
Sample : HAP1 cells
Lane Description : SM: 10% starting material UB: 10% unbound fraction IP: immunoprecipitate
Figure Note : Antibody-bead conjugates were prepared by adding 2.0 μg of antibody to 500 μL of IP Lysis Buffer, together with 30 μL of protein A-Sepharose beads. One ml aliquots at 1.0 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4ºC. Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-STAT5B antibody [GT1160] used in Western Blot (WB). GTX08967
Date : Anonymous submitted on 09-Nov-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and STAT5B KO cells
Amount used : 20 μg
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399