APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Product Note
IP/MS validation was supported by references (PMID:30377420)
Predict Reactivity
Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.23 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human STAT5B. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886571
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
signal transducer and activator of transcription 5B , STAT5
Cellular Localization
Cytoplasm , Nucleus
Background
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX132113 ICC/IF Image
STAT5B antibody detects STAT5B protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: STAT5B protein stained by STAT5B antibody (GTX132113) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX132113 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT5B antibody (GTX132113) diluted at1:1000.
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GTX132113 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT5B antibody (GTX132113) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
STAT5B antibody
Cat. No. GTX132113
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Rating
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( Average 5 based on 1 users reviews)
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Application
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Immunoprecipitation(IP)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 09-Nov-2022
Application Tested :
IP
Sample Species :
Hu
Sample :
HAP1 cells
Lane Description :
SM: 10% starting material
UB: 10% unbound fraction
IP: immunoprecipitate
Figure Note :
Antibody-bead conjugates were prepared by adding 2.0 μg of antibody to 500 μL of IP Lysis Buffer, together with 30 μL of protein A-Sepharose beads. One ml aliquots at 1.0 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4ºC.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
