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STAT5B antibody

Anti-STAT5B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132113
Anti-STAT5B antibody used in Western Blot (WB). GTX132113

Cat No. GTX132113

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377420)

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human STAT5B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

signal transducer and activator of transcription 5B,STAT5

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-STAT5B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132113

GTX132113 ICC/IF Image

STAT5B antibody detects STAT5B protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: STAT5B protein stained by STAT5B antibody (GTX132113) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-STAT5B antibody used in Western Blot (WB). GTX132113

GTX132113 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT5B antibody (GTX132113) diluted at1:1000.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169