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STAT6 antibody

Orthogonal IP/MS
Anti-STAT6 antibody used in Immunoprecipitation (IP). GTX113273
Anti-STAT6 antibody used in Western Blot (WB). GTX113273
Anti-STAT6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113273
Anti-STAT6 antibody used in IHC (Paraffin sections) (IHC-P). GTX113273
Anti-STAT6 antibody used in IHC (Paraffin sections) (IHC-P). GTX113273

Cat. No. GTX113273

Reference 5
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all STAT6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Product Note

IP/MS validation was supported by references (PMID:30377411)

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human STAT6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721049

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal transducer and activator of transcription 6 , D12S1644 , IL-4-STAT , STAT6B , STAT6C

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-STAT6 antibody used in Immunoprecipitation (IP). GTX113273

GTX113273 IP Image

STAT6 antibody immunoprecipitates STAT6 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 50 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of STAT6 protein by 2 μg of STAT6 antibody (GTX113273) 10% SDS-PAGE The immunoprecipitated STAT6 protein was detected by STAT6 antibody (GTX113273) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-STAT6 antibody used in Western Blot (WB). GTX113273

GTX113273 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with STAT6 antibody (GTX113273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-STAT6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113273

GTX113273 ICC/IF Image

STAT6 antibody detects STAT6 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: STAT6 stained by STAT6 antibody (GTX113273) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-STAT6 antibody used in IHC (Paraffin sections) (IHC-P). GTX113273

GTX113273 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using STAT6 antibody(GTX113273) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-STAT6 antibody used in IHC (Paraffin sections) (IHC-P). GTX113273

GTX113273 IHC-P Image

STAT6 antibody detects STAT6 protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
STAT6 antibody (GTX113273) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Traxel S et al. Cancer Immunol Immunother 2021; Support of BCP-ALL-cells by autologous bone marrow Th-cells involves induction of AID expression but not widespread AID off-target mutagenesis.
Application : WB
Reactivity : Human
Jung MJ et al. J Breast Cancer 2019; 22 (2) : 326-335 Solitary Fibrous Tumor of Breast with Anaplastic Areas (Malignant Solitary Fibrous Tumor): A Case Report with Review of Literature.
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Hynds RE et al. PLoS One 2018; 13 (5) : e0197129 Cross-talk between human airway epithelial cells and 3T3-J2 feeder cells involves partial activation of human MET by murine HGF.
Application : WB
Reactivity : Human
Xu YM et al. Oncotarget 2016; 7 (5) : 6146-58 Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.
Reactivity : Human
Submit a Reference

REVIEW

STAT6 antibody Cat. No. GTX113273

Rating
( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-STAT6 antibody used in IHC (Paraffin sections) (IHC-P). GTX113273
Date : Anonymous submitted on 05-Jan-2017
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Lung tumor/solitary fibrous tumor
Blocking : Protein Blood Serum-free (DAKO X0909), 25°C, 0.3Hr
Fixation : 20% Formalin Neutral Buffer Solution
Permeabilization /Antigen retrieval : 10 mM sodium citrate buffer pH 6.0
Primary Antibody : 1:120, 37°C, 1Hr
Figure Note : The MW image was treated only by microwave and the MW+pep image was treated by both microwave and pepsin.
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169