GeneTex
United States (US)

STEAP1 antibody [J2D2]

GTX53786_IHC_18121410_156.jpg
GTX53786_ICCIF_18121410_624.jpg
GTX53786_WB_18121410_323.jpg

Cat No. GTX53786

Host Mouse
Clonality Monoclonal
Clone Name J2D2
Isotype IgG2b
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Rat
APPLICATION

Application Note

The antibody has been tested by ELISA, Western blot, immunofluorescence and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000~1:2,000, immunofluorescence 1:100 ~1:300 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000, Immunofluorescence 1:100 and Immunohistochemistry analysis is 1:50.

Calculated MW

40 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human STEAP1 (1-70aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PRSS24 Antibody , six transmembrane epithelial antigen of the prostate 1 Antibody , STEAP Antibody

Background

This gene is predominantly expressed in prostate tissue, and is found to be upregulated in multiple cancer cell lines. The gene product is predicted to be a six-transmembrane protein, and was shown to be a cell surface antigen significantly expressed at cell-cell junctions. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX53786_IHC_18121410_156.jpg

GTX53786 IHC-P Image

IHC-P analysis of human breast cancer and prostate hyperplasia tissue using STEAP1 antibody at a dilution of 1:50.

GTX53786_ICCIF_18121410_624.jpg

GTX53786 ICC/IF Image

ICC/IF analysis of human LNCap cells using STEAP1 antibody.

GTX53786_WB_18121410_323.jpg

GTX53786 WB Image

WB analysis of LNcap and Rat testis lysates (30ug per lane) using STEAP1 antibody at a dilution of 1:500.

Package List Price ($)
$ 319