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STEAP1 antibody [J2D2]

Anti-STEAP1 antibody [J2D2] used in IHC (Paraffin sections) (IHC-P). GTX53786
Anti-STEAP1 antibody [J2D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53786
Anti-STEAP1 antibody [J2D2] used in Western Blot (WB). GTX53786

Cat. No. GTX53786

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 J2D2

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Rat
Package
100 μl ($399)
See all STEAP1 products

APPLICATION

Application Note

The antibody has been tested by ELISA, Western blot, immunofluorescence and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000~1:2,000, immunofluorescence 1:100 ~1:300 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000, Immunofluorescence 1:100 and Immunohistochemistry analysis is 1:50.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human STEAP1 (1-70aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

STEAP family member 1 , PRSS24 , STEAP

Cellular Localization

Endosome membrane

Background

This gene is predominantly expressed in prostate tissue, and is found to be upregulated in multiple cancer cell lines. The gene product is predicted to be a six-transmembrane protein, and was shown to be a cell surface antigen significantly expressed at cell-cell junctions. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-STEAP1 antibody [J2D2] used in IHC (Paraffin sections) (IHC-P). GTX53786

GTX53786 IHC-P Image

IHC-P analysis of human breast cancer and prostate hyperplasia tissue using STEAP1 antibody at a dilution of 1:50.

Anti-STEAP1 antibody [J2D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53786

GTX53786 ICC/IF Image

ICC/IF analysis of human LNCap cells using STEAP1 antibody.

Anti-STEAP1 antibody [J2D2] used in Western Blot (WB). GTX53786

GTX53786 WB Image

WB analysis of LNcap and Rat testis lysates (30ug per lane) using STEAP1 antibody at a dilution of 1:500.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399