APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| Assay dependent |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Mouse Thymus Tissue Lysate(GTX24028) , Human Spleen Tissue Lysate(GTX27921) , Human Spleen Tissue Slide
Product Note
At least two isoforms of STIM1 are known to exist; this antibody will detect only the larger form. This STIM1 antibody is predicted to have no cross-reactivity to STIM2.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
STIM1 antibody was raised against a 24 amino acid synthetic peptide from near the carboxy terminus of human STIM1.The immunogen is located within the last 50 amino acids of STIM1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
stromal interaction molecule 1 , D11S4896E , GOK , IMD10 , STRMK , TAM , TAM1
Cellular Localization
Cell membrane; Single-pass type I membrane protein,Cytoplasm, cytoskeleton
Background
This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene.[provided by RefSeq, Jan 2009]
Database
Research Area
DATA IMAGES
|
GTX31757 IHC-P Image
IHC-P analysis of mouse spleen tissue using GTX31757 STIM1 antibody.
Working concentration : 5 μg/ml
|
|
|
GTX31757 WB Image
WB analysis of mouse thymus tissue lysate using GTX31757 STIM1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
|
|
|
GTX31757 IHC-P Image
IHC-P analysis of human spleen tissue using GTX31757 STIM1 antibody.
Working concentration : 2.5 μg/ml
|
|
|
GTX31757 IHC-P Image
IHC-P analysis of mouse spleen tissue using GTX31757 STIM1 antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml
|
|
|
GTX31757 ICC/IF Image
ICC/IF analysis of HEK293 cells using GTX31757 STIM1 antibody.
Red : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml
|
REFERENCE
There are currently no references for STIM1 antibody (GTX31757). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for STIM1 antibody (GTX31757). Be the first to share your experience with this product.
