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STIM1 antibody

Anti-STIM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31757
Anti-STIM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31757
Anti-STIM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31757
Anti-STIM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31757
Anti-STIM1 antibody used in Western Blot (WB). GTX31757

Cat No. GTX31757

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ICC/IF Assay dependent
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

Mouse Thymus Tissue Lysate(GTX24028) , Human Spleen Tissue Lysate(GTX27921) , Human Spleen Tissue Slide

Specificity/Sensitivity

At least two isoforms of STIM1 are known to exist; this antibody will detect only the larger form. This STIM1 antibody is predicted to have no cross-reactivity to STIM2.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

STIM1 antibody was raised against a 24 amino acid synthetic peptide from near the carboxy terminus of human STIM1.The immunogen is located within the last 50 amino acids of STIM1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

stromal interaction molecule 1 , D11S4896E , GOK , IMD10 , STRMK , TAM , TAM1

Cellular Localization

Cell membrane; Single-pass type I membrane protein,Cytoplasm, cytoskeleton

Background

This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene.[provided by RefSeq, Jan 2009]

Database

Research Area

DATA IMAGES

Anti-STIM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31757

GTX31757 ICC/IF Image

ICC/IF analysis of HEK293 cells using GTX31757 STIM1 antibody.
Red : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml

Anti-STIM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31757

GTX31757 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX31757 STIM1 antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml

Anti-STIM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31757

GTX31757 IHC-P Image

IHC-P analysis of human spleen tissue using GTX31757 STIM1 antibody.
Working concentration : 2.5 μg/ml

Anti-STIM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31757

GTX31757 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX31757 STIM1 antibody.
Working concentration : 5 μg/ml

Anti-STIM1 antibody used in Western Blot (WB). GTX31757

GTX31757 WB Image

WB analysis of mouse thymus tissue lysate using GTX31757 STIM1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339