APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.45 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide CHSEDEKLSFEAVR, corresponding to amino acid residues 56-69 (STIM1 expressed on the plasma membrane: Extracellular, N-terminus st IM1 expressed on th e ER: Luminal, N-terminus) of human STIM1 (Accession : Q13586).
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
RRID
AB_2887895
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
stromal interaction molecule 1 , D11S4896E , GOK , IMD10 , STRMK , TAM , TAM1
Cellular Localization
Cell membrane; Single-pass type I membrane protein,Cytoplasm, cytoskeleton
Background
This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]
Database
Research Area
DATA IMAGES
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GTX54855 IHC-P Image
IHC-P analysis of rat stomach tissue using GTX54855 STIM1 antibody. STIM1 is expressed in the parietal cells of the gastric mucosa (arrows). Hematoxilin is used as the counterstain.
Dilution : 1:100
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GTX54855 WB Image
WB analysis of RBL (lanes 1 and 4), HL-60 (lanes 2 and 5), and Jurkat (lanes 3 and 6) cell lysates using GTX54855 STIM1 antibody preincubated with or without immunogen peptide.
Dilution : 1:1000
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GTX54855 LCI Image
Live cell imaging analysis of live rat RBL cells using GTX54855 STIM1 antibody.
Panel A : Primary antibody (Orange)
Panel B : Hoechst 33342 for nuclear staining (blue)
Panel C : Merged images of Panel A and B
Dilution : 1:50
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GTX54855 FACS Image
FACS analysis of RBL (A) and Jurkat (B) cells using GTX54855 STIM1 antibody.
Black : Unstained cell
Green : Cell staining with primary antibody
Antibody amount : 5-10 μg antibody / 0.5-1 x 10⁶ cells
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REFERENCE
REVIEW
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