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STIM2 antibody

Anti-STIM2 antibody used in Western Blot (WB). GTX54768
Anti-STIM2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54768
Anti-STIM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX54768

Cat. No. GTX54768

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
50 μl ($639)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide SAEKQWEVPDTASEC, corresponding to amino acid residues 583-597 (Intracellular, C-terminus) of human STIM2 (Accession  : Q9P246).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

stromal interaction molecule 2

Cellular Localization

Endoplasmic reticulum membrane

Background

This gene is a member of the stromal interaction molecule (STIM) family and likely arose, along with related family member STIM1, from a common ancestral gene. The encoded protein functions to regulate calcium concentrations in the cytosol and endoplasmic reticulum, and is involved in the activation of plasma membrane Orai Ca(2+) entry channels. This gene initiates translation from a non-AUG (UUG) start site. A signal peptide is cleaved from the resulting protein. Multiple transcript variants result from alternative splicing. [provided by RefSeq, Dec 2009]

Database

DATA IMAGES

Anti-STIM2 antibody used in Western Blot (WB). GTX54768

GTX54768 WB Image

WB analysis of rat brain (lanes 1, 3) and RBL cell (lanes 2, 4) lysates using GTX54768 STIM2 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-STIM2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54768

GTX54768 ICC/IF Image

ICC/IF analysis of PFA-fixed RBL cells using GTX54768 STIM2 antibody.
Panel A,D : Primary antibody
Panel B,E : DNA dye Hoechst 33342
Panel C,F : Merged images of panels A and B
Dilution : 1:100

Anti-STIM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX54768

GTX54768 IHC-P Image

IHC-P analysis of rat lung tissue using GTX54768 STIM2 antibody. Hematoxilin is used as the counterstain.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639