GeneTex

  • Country / Location Selection

United States (US)

STIM2 antibody

Anti-STIM2 antibody used in Western Blot (WB). GTX85440
Anti-STIM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85440
Anti-STIM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85440

Cat. No. GTX85440

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all STIM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

A20 Cell Lysate(GTX27180) , Human Spleen Tissue Lysate(GTX27921) , Human Spleen Tissue Slide

Product Note

This antibody is specific for STIM2 Internal

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

STIM2 antibody was raised against a 17 amino acid synthetic peptide from near the center of human STIM2.The immunogen is located within amino acids 140 - 190 of STIM2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10731282

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

stromal interaction molecule 2

Cellular Localization

Endoplasmic reticulum membrane

Background

In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++-release-activated Ca++ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry. At least three isoforms of STIM2 are known to exist. This STIM2 antibody is predicted to have no cross-reactivity to STIM1.

Database

DATA IMAGES

Anti-STIM2 antibody used in Western Blot (WB). GTX85440

GTX85440 WB Image

WB analysis of A-20 cell lysate using GTX85440 STIM2 antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

Anti-STIM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85440

GTX85440 IHC-P Image

IHC-P analysis of human spleen tissue using GTX85440 STIM2 antibody.
Working concentration : 20 μg/ml

Anti-STIM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85440

GTX85440 IHC-P Image

IHC-P analysis of human spleen tissue using GTX85440 STIM2 antibody.
Working concentration : 2.5 μg/ml

REFERENCE

Application Reference

Morin G et al. Hum Mutat 2014; Gain-of-Function mutation in STIM1 (p.R304W) is Associated with Stormorken Syndrome.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for STIM2 antibody (GTX85440). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399