Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1 μg/mL |
5 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
A-20 Cell Slide(GTX25541) , A20 Cell Lysate(GTX27180) , Jurkat Cell Lysate(GTX27899) , K562 Cell Lysate(GTX27911)
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
MPYS antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human MPYS.The immunogen is located within amino acids 260 - 310 of MPYS.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
Knockdown/Knockout validation was supported by references (PMID: 35538059).
Synonyms
transmembrane protein 173 , ERIS , MITA , MPYS , NET23 , SAVI , STING , STING-beta , hMITA , hSTING
Cellular Localization
Endoplasmic reticulum membrane,Mitochondrion outer membrane,Cell membrane,Cytoplasm
Background
MPYS is a recently identified plasma membrane tetraspanner that is associated with major histocompatibility complex class II (MHC-II) and mediates its transduction of apoptotic signals. It has also been found to be associated with VISA, a mitochondrial protein that acts as an adaptor in virus-triggered signaling. MPYS also interacts with IRF3 and recruits the kinase TBK1 to the VISA-associated complex, acting as a critical mediator of virus-triggered IRF3 activation and interferon (IFN) expression. It is thought that the binding of nucleic acid to the innate immune protein RIG-I causes complex formation between RIG-I, VISA, and MPYS. This complex then recruits TBK1 to phosphorylate IRF3 which then directly activates IFN transcription. At least three isoforms of MPYS are known to exist.
Database
Research Area