Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using STIP1(GTX103019) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using STIP1(GTX103019) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: K562
D: THP-1
7.5% SDS PAGE
GTX103019 diluted at 1:10000