GeneTex
United States (US)

STIP1 antibody

GTX103019_40688_WB_19011506_247.jpg
GTX103019_40688_IFA_19011506_384.jpg
GTX103019_40688_IHC_19011506_913.jpg

Cat No. GTX103019

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , THP-1

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human STIP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Stress Induced Phosphoprotein 1,Hel-S-94N,Hop,Ief-Ssp-3521,P60,Sti1,Sti1L,Stip1

Cellular Localization

Cytoplasm , Nucleus

Background

STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM]

Database

DATA IMAGES
GTX103019_40688_WB_19011506_247.jpg

GTX103019 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: K562
D: THP-1
7.5% SDS PAGE
GTX103019 diluted at 1:10000

GTX103019_40688_IFA_19011506_384.jpg

GTX103019 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using STIP1(GTX103019) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX103019_40688_IHC_19011506_913.jpg

GTX103019 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using STIP1(GTX103019) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169