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STIP1 antibody

Anti-STIP1 antibody used in Western Blot (WB). GTX103019
Anti-STIP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103019
Anti-STIP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103019

Cat. No. GTX103019

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , THP-1

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human STIP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176960

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

stress induced phosphoprotein 1 , HEL-S-94n , HOP , IEF-SSP-3521 , P60 , STI1 , STI1L

Cellular Localization

Cytoplasm , Nucleus

Background

STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-STIP1 antibody used in Western Blot (WB). GTX103019

GTX103019 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: K562
D: THP-1
7.5% SDS PAGE
GTX103019 diluted at 1:10000

Anti-STIP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103019

GTX103019 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using STIP1(GTX103019) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-STIP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103019

GTX103019 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using STIP1(GTX103019) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

There are currently no references for STIP1 antibody (GTX103019). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for STIP1 antibody (GTX103019). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169