STIP1 antibody

Name: STIP1 antibody
Brand: GeneTex
SKU: GTX103068
Price: 399.00 USD

Anti-STIP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103068

Anti-STIP1 antibody used in Western Blot (WB). GTX103068

Anti-STIP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103068

Cat. No. GTX103068

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all STIP1 products

View product citations for antibody GTX103068 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	Assay dependent

Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , HepG2 , Molt-4

Predict Reactivity

Bovine, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human STIP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952047

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

stress induced phosphoprotein 1 , HEL-S-94n , HOP , IEF-SSP-3521 , P60 , STI1 , STI1L

Cellular Localization

Cytoplasm , Nucleus

Background

STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM]

Database

Gene ID: 10963 STIP1
UniProt: P31948 STIP1

Research Area

DATA IMAGES

GTX103068 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using STIP1(GTX103068) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103068 WB Image

Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
B:MOLT4(GTX27912)
7.5% SDS PAGE
GTX103068 diluted at 1:5000

GTX103068 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using STIP1(GTX103068) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX103068 WB Image

STIP1 antibody detects STIP1 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
STIP1 antibody (GTX103068) dilution: 1:1000