GeneTex
United States (US)

STK25 antibody [N1C1]

GTX107634_40016_IFA_19011506_543.jpg
GTX107634_WB_19011506_198.jpg
GTX107634_IHC_19011506_444.jpg

Cat No. GTX107634

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine, Dog, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human STK25. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Serine/Threonine Kinase 25,Sok1,Ysk1,Stk25

Cellular Localization

Cytoplasm , Golgi apparatus

Background

Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration.

Database

Research Area

DATA IMAGES
GTX107634_40016_IFA_19011506_543.jpg

GTX107634 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using STK25(GTX107634) antibody at 1:200 dilution.

GTX107634_WB_19011506_198.jpg

GTX107634 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa S3
10% SDS PAGE
GTX107634 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107634_IHC_19011506_444.jpg

GTX107634 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using STK25(GTX107634) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169