GeneTex
United States (US)

STK3 antibody

GTX107417_39911_WB_M_19011506_655.jpg
GTX107417_39911_IFA_19011506_376.jpg
GTX107417_39911_WB_B_19011506_535.jpg
GTX107417_39911_IHC_19011506_154.jpg

Cat No. GTX107417

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

293T transfected STK3 , , NIH-3T3 , JC

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human STK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Serine/Threonine Kinase 3,Krs1,Mst2,Stk3

Cellular Localization

Cytoplasm , Nucleus

Background

Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (MIM 134638) (Taylor et al., 1996 [PubMed 8816758]; Lee et al., 2001 [PubMed 11278283]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX107417_39911_WB_M_19011506_655.jpg

GTX107417 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX107417 diluted at 1:5000

GTX107417_39911_IFA_19011506_376.jpg

GTX107417 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using STK3(GTX107417) antibody at 1:500 dilution.

GTX107417_39911_WB_B_19011506_535.jpg

GTX107417 WB Image

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: STK3 transfected 293T lysates
7.5% SDS PAGE
GTX107417 diluted at 1:5000

GTX107417_39911_IHC_19011506_154.jpg

GTX107417 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using STK3(GTX107417) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169