APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
THP-1 , NCI-H929 , STK3 transfected 293T , 293T , A431
Predict Reactivity
Mouse, Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human STK3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11166238
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
serine/threonine kinase 3 , KRS1 , MST2
Cellular Localization
Cytoplasm , Nucleus
Background
Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (MIM 134638) (Taylor et al., 1996 [PubMed 8816758]; Lee et al., 2001 [PubMed 11278283]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX111494 IP Image
STK3 antibody immunoprecipitates STK3 protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of STK3 protein by 4 μg STK3 antibody (GTX111494)
7.5 % SDS-PAGE
The immunoprecipitated STK3 protein was detected by STK3 antibody (GTX111494) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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GTX111494 WB Image
STK3 antibody detects STK3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with STK3 antibody (GTX111494) diluted by 1:1000.
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GTX111494 WB Image
Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: STK3 transfected 293T lysates
7.5% SDS PAGE
GTX111494 diluted at 1:5000
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GTX111494 WB Image
Sample (30 ug of whole cell lysate)
A: THP-1
B: NCI-H929
7.5% SDS PAGE
GTX111494 diluted at 1:1000
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REFERENCE
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REVIEW
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