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STK40 antibody [N3C3]

Overexpression
Anti-STK40 antibody [N3C3] used in Western Blot (WB). GTX111337
Anti-STK40 antibody [N3C3] used in Western Blot (WB). GTX111337
Anti-STK40 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111337

Cat. No. GTX111337

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all STK40 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

HeLa , HepG2 , Myc-DDK-tagged serine/threonine kinase 40-transfected 293T cells

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human STK40. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952079

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serine/threonine kinase 40 , SHIK , SgK495

Cellular Localization

Nucleus , Cytoplasm

Background

May be a negative regulator of NF-kappa-B and p53-mediated gene transcription.

Database

Research Area

DATA IMAGES

Anti-STK40 antibody [N3C3] used in Western Blot (WB). GTX111337

GTX111337 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
10% SDS PAGE
GTX111337 diluted at 1:1000

Anti-STK40 antibody [N3C3] used in Western Blot (WB). GTX111337

GTX111337 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with serine/threonine kinase 40 antibody [N3C3] (GTX111337) diluted at 1:5000.

Anti-STK40 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111337

GTX111337 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using serine/threonine kinase 40(GTX111337) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169