Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using serine/threonine kinase 40(GTX111337) antibody at 1:200 dilution.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with serine/threonine kinase 40 antibody [N3C3] (GTX111337) diluted at 1:5000.

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
10% SDS PAGE
GTX111337 diluted at 1:1000