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STUB1 antibody [C1C3]

Anti-STUB1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX109676
Anti-STUB1 antibody [C1C3] used in Western Blot (WB). GTX109676
Anti-STUB1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX109676
Anti-STUB1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109676

Cat. No. GTX109676

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all STUB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

293T , HepG2

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human STUB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952087

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

STIP1 homology and U-box containing protein 1 , CHIP , HSPABP2 , NY-CO-7 , SCA48 , SCAR16 , SDCCAG7 , UBOX1

Cellular Localization

Cytoplasm

Background

STUB1, or CHIP, is a ubiquitin ligase/cochaperone that participates in protein quality control by targeting a broad range of chaperone protein substrates for degradation (Min et al., 2008 [PubMed 18411298]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-STUB1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX109676

GTX109676 IHC-P Image

Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using STUB1(GTX109676) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-STUB1 antibody [C1C3] used in Western Blot (WB). GTX109676

GTX109676 WB Image

Sample(30 ug whole cell lysate)
A:293T
B:Hep G2 (GTX27900)
10% SDS PAGE
GTX109676 diluted at 1:1000

Anti-STUB1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX109676

GTX109676 IHC-P Image

STUB1 antibody [C1C3] detects STUB1 protein at cytoplasm on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
STUB1 antibody [C1C3] (GTX109676) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-STUB1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109676

GTX109676 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CHIP (GTX109676) antibody at 1:200 dilution.

REFERENCE

Application Reference

Lin HY et al. Biomed Pharmacother 2022; 145 : 112431 Epigenetic therapy combination of UNC0638 and CI-994 suppresses breast cancer via epigenetic remodeling of BIRC5 and GADD45A.
Application : WB
Reactivity : Human
Salim C et al. J Orofac Orthop 2021; Role of chaperone-assisted selective autophagy (CASA) in mechanical stress protection of periodontal ligament cells.
Application : WB
Reactivity : Human
Lai HH et al. Biochem Biophys Res Commun 2018; 506 (1) : 87-93 role of dicer in regulating * resistance of colon cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169