APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:5000-1:20000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2
Predict Reactivity
Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human STUB1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11178641
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
STIP1 homology and U-box containing protein 1 , CHIP , HSPABP2 , NY-CO-7 , SCA48 , SCAR16 , SDCCAG7 , UBOX1
Cellular Localization
Cytoplasm
Background
STUB1, or CHIP, is a ubiquitin ligase/cochaperone that participates in protein quality control by targeting a broad range of chaperone protein substrates for degradation (Min et al., 2008 [PubMed 18411298]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX122827 WB Image
Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE GTX122827 diluted at 1:10000
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GTX122827 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human breast cancer, using STUB1(GTX122827) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX122827 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with STUB1 antibody [N1C3] (GTX122827) diluted at 1:10000.
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GTX122827 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with STUB1 antibody [N1C3] (GTX122827) diluted at 1:10000.
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REFERENCE
There are currently no references for STUB1 antibody [N1C3] (GTX122827). Be the first to share your publications with this product.
REVIEW
STUB1 antibody [N1C3]
Cat. No. GTX122827
Rating
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( Average 5 based on 1 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 24-Oct-2014
Application Tested :
WB
Sample Species :
Hu
Amount used :
20μg
Blocking :
1% milk in PBST, 25°C, 1Hr
Primary Antibody :
1:5000, 25°C, 2Hr
Lane Description :
Lane 1: BT-474 cells, Lane 2: HCC1937 cells, Lane 3: Hs578T cells, Lane 4: MCF7 cells, Lane 5: MDA-MB-231 cells, Lane 6: MDA-MB-361 cells, Lane 7: MDA-MB-453 cells, Lane 8: T-47D cells, Lane 9: ZR-75-1 cells, Lane 10: HeLa cells, Lane 11: U2OS cells, Lane 12: 293T cells