Wild-type (WT) and SUCLA2 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLA2 antibody [N2C3] (GTX109728) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
B: Molt-4 (GTX27912)
10% SDS PAGE
GTX109728 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SUCLA2(GTX109728) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (20 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX109728 diluted at 1:20000

SUCLA2 antibody [N2C3] detects SUCLA2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: SUCLA2 protein stained by SUCLA2 antibody [N2C3] (GTX109728) diluted at 1:500.
Blue: Hoechst 33342 staining.

SUCLA2 antibody [N2C3] detects SUCLA2 protein at cytoplasm on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
SUCLA2 antibody [N2C3] (GTX109728) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min