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SUCLG1 antibody [HL2387]

Recombinant antibody
Anti-SUCLG1 antibody [HL2387] used in IHC (Paraffin sections) (IHC-P). GTX638596
Anti-SUCLG1 antibody [HL2387] used in IHC (Paraffin sections) (IHC-P). GTX638596
Anti-SUCLG1 antibody [HL2387] used in IHC (Paraffin sections) (IHC-P). GTX638596
Anti-SUCLG1 antibody [HL2387] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638596
Anti-SUCLG1 antibody [HL2387] used in Western Blot (WB). GTX638596
Anti-SUCLG1 antibody [HL2387] used in Western Blot (WB). GTX638596
Anti-SUCLG1 antibody [HL2387] used in Western Blot (WB). GTX638596

Cat. No. GTX638596

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2387

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SUCLG1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length of human SUCLG1 protein.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

succinate-CoA ligase alpha subunit , GALPHA , MTDPS9 , SUCLA1

Cellular Localization

Mitochondrion

Background

This gene encodes the alpha subunit of the heterodimeric enzyme succinate coenzyme A ligase. This enzyme is targeted to the mitochondria and catalyzes the conversion of succinyl CoA and ADP or GDP to succinate and ATP or GTP. Mutations in this gene are the cause of the metabolic disorder fatal infantile lactic acidosis and mitochondrial DNA depletion. [provided by RefSeq, Feb 2010]

Database

Research Area

DATA IMAGES

Anti-SUCLG1 antibody [HL2387] used in IHC (Paraffin sections) (IHC-P). GTX638596

GTX638596 IHC-P Image

SUCLG1 antibody [HL2387] detects SUCLG1 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
SUCLG1 stained by SUCLG1 antibody [HL2387] (GTX638596) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SUCLG1 antibody [HL2387] used in IHC (Paraffin sections) (IHC-P). GTX638596

GTX638596 IHC-P Image

SUCLG1 antibody [HL2387] detects SUCLG1 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
SUCLG1 stained by SUCLG1 antibody [HL2387] (GTX638596) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SUCLG1 antibody [HL2387] used in IHC (Paraffin sections) (IHC-P). GTX638596

GTX638596 IHC-P Image

SUCLG1 antibody [HL2387] detects SUCLG1 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissue.
SUCLG1 stained by SUCLG1 antibody [HL2387] (GTX638596) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SUCLG1 antibody [HL2387] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638596

GTX638596 ICC/IF Image

SUCLG1 antibody [HL2387] detects SUCLG1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: SUCLG1 stained by SUCLG1 antibody [HL2387] (GTX638596) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SUCLG1 antibody [HL2387] used in Western Blot (WB). GTX638596

GTX638596 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLG1 antibody [HL2387] (GTX638596) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SUCLG1 antibody [HL2387] used in Western Blot (WB). GTX638596

GTX638596 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLG1 antibody [HL2387] (GTX638596) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SUCLG1 antibody [HL2387] used in Western Blot (WB). GTX638596

GTX638596 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLG1 antibody [HL2387] (GTX638596) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations