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SUCLG2 antibody

Anti-SUCLG2 antibody used in Western Blot (WB). GTX107002
Anti-SUCLG2 antibody used in Western Blot (WB). GTX107002
Anti-SUCLG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX107002
Anti-SUCLG2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107002
Anti-SUCLG2 antibody used in Western Blot (WB). GTX107002

Cat No. GTX107002

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse liver

Predict Reactivity

Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SUCLG2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Succinate-Coa Ligase Gdp-Forming Beta Subunit,G-Scs,Gbeta,Gtpscs,Suclg2

Cellular Localization

Mitochondrion

Background

This gene encodes a GTP-specific beta subunit of succinyl-CoA synthetase. Succinyl-CoA synthetase catalyzes the reversible reaction involving the formation of succinyl-CoA and succinate. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 5 and 12. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-SUCLG2 antibody used in Western Blot (WB). GTX107002

GTX107002 WB Image

Wild-type (WT) and SUCLG2 knockout (KO) 293T cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLG2 antibody (GTX107002) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SUCLG2 antibody used in Western Blot (WB). GTX107002

GTX107002 WB Image

Sample (20 µg of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX107002 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SUCLG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX107002

GTX107002 IHC-P Image

SUCLG2 antibody detects SUCLG2 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
SUCLG2 antibody (GTX107002) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SUCLG2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107002

GTX107002 ICC/IF Image

SUCLG2 antibody detects SUCLG2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: SUCLG2 protein stained by SUCLG2 antibody (GTX107002) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-SUCLG2 antibody used in Western Blot (WB). GTX107002

GTX107002 WB Image

Sample(30 µg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: HepG2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
10% SDS PAGE
GTX107002 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169