GeneTex
United States (US)

SUGT1 antibody [N1C3]

GTX105358_IFA_19011506_361.jpg
GTX105358_39904_20141121_WB_19011506_980.jpg

Cat No. GTX105358

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

293T , A431 , HeLa

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SUGT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

SGT1 antibody, SUGT1 antibody, "suppressor of G2 allele of SKP1, S. cerevisiae, homolog of antibody", putative 40-6-3 protein antibody, suppressor of G2 allele of SKP1 homolog antibody, SGT1B protein antibody, "SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae) antibody"

Background

This gene is homologous to the yeast gene SGT1, which encodes a protein involved in kinetochore function and required for the G1/S and G2/M transitions. Complementation studies suggest that the human protein has similar functions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105358_IFA_19011506_361.jpg

GTX105358 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SUGT1(GTX105358) antibody at 1:200 dilution.

GTX105358_39904_20141121_WB_19011506_980.jpg

GTX105358 WB Image

SUGT1 antibody detects SUGT1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SUGT1 antibody (GTX105358) diluted by 1:3000.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169