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SULF2 antibody, Internal

Anti-SULF2 antibody, Internal used in Western Blot (WB). GTX47559

Cat No. GTX47559

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX47559-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_061325.1; NP_940998.1)

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DGRVYHVGLGDAAQPR , from the internal region of the protein sequence according to NP_061325.1; NP_940998.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Sulfatase 2 , Hsulf-2 , Sulf2

Cellular Localization

Also localized on the cell surface.

Background

Heparan sulfate proteoglycans (HSPGs) act as coreceptors for numerous heparin-binding growth factors and cytokines and are involved in cell signaling. Heparan sulfate 6-O-endosulfatases, such as SULF2, selectively remove 6-O-sulfate groups from heparan sulfate. This activity modulates the effects of heparan sulfate by altering binding sites for signaling molecules (Dai et al., 2005 [PubMed 16192265]).[supplied by OMIM, Mar 2008]

Database

DATA IMAGES

Anti-SULF2 antibody, Internal used in Western Blot (WB). GTX47559

GTX47559 WB Image

WB analysis of human ovary lysate with (B) and without (A) blocking with the immunising peptide using GTX47559 SULF2 antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 369