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SUMO1 antibody [4D12]

Anti-SUMO1 antibody [4D12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00701
Anti-SUMO1 antibody [4D12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00701
Anti-SUMO1 antibody [4D12] used in Western Blot (WB). GTX00701

Cat. No. GTX00701

Reference 2

Host

 Rat

Clonality

 Monoclonal

Clone Name

 4D12

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-Fr, ELISA

Reactivity

 Human, Mouse, Rat, Simian
Package
100 μg ($399)
See all SUMO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC-Fr 1:100
ELISA Assay dependent
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant GST-fused human SUMO1 (full length)

Purification

Purified IgG
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

small ubiquitin like modifier 1 , DAP1 , GMP1 , OFC10 , PIC1 , SENP2 , SMT3 , SMT3C , SMT3H3 , UBL1

Cellular Localization

Nucleus speckle,Cytoplasm,Cell membrane,Nucleus

Background

This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SUMO1 antibody [4D12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00701

GTX00701 ICC/IF Image

ICC/IF analysis of C-33A cells using GTX00701 SUMO1 antibody [4D12].
SUMO-1 colocalizes with SUMO2/3.
Left : anti-SUMO-1 antibody (4D12)
Middle : anti-SUMO2/3 antibody (3H12)
Right : Merged image
Dilution : 10 μg/ml

Anti-SUMO1 antibody [4D12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00701

GTX00701 ICC/IF Image

ICC/IF analysis of mouse primary culture neurons using GTX00701 SUMO1 antibody [4D12].
Dilution : 10 μg/ml

Anti-SUMO1 antibody [4D12] used in Western Blot (WB). GTX00701

GTX00701 WB Image

WB analysis of HeLa whole cell lysate using GTX00701 SUMO1 antibody [4D12].
The 80 kDa band would be SUMO-RanGAP.
Dilution : 1 μg/ml

REFERENCE

Clone Reference

Saitoh N et al. Exp Cell Res. 2006; In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies.
Uchimura Y et al. J Biol Chem. 2006; Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399