APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human SUMO1 (NP_003343.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
small ubiquitin like modifier 1 , DAP1 , GMP1 , OFC10 , PIC1 , SENP2 , SMT3 , SMT3C , SMT3H3 , UBL1
Cellular Localization
Nucleus speckle,Cytoplasm,Cell membrane,Nucleus
Background
This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX55812 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX55812 SUMO1 antibody.
Blue : DAPI
Dilution : 1:100
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GTX55812 IHC-P Image
IHC-P analysis of rat brain tissue using GTX55812 SUMO1 antibody.
Dilution : 1:200
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GTX55812 ICC/IF Image
ICC/IF analysis of C6 cells using GTX55812 SUMO1 antibody.
Blue : DAPI
Dilution : 1:100
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GTX55812 WB Image
WB analysis of 293T cell lysate using GTX55812 SUMO1 antibody.
Dilution : 1:500
Loading : 25μg per lane
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GTX55812 IHC-P Image
IHC-P analysis of human lung cancer tissue using GTX55812 SUMO1 antibody.
Dilution : 1:200
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GTX55812 IHC-P Image
IHC-P analysis of mouse testis tissue using GTX55812 SUMO1 antibody.
Dilution : 1:200
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GTX55812 ICC/IF Image
ICC/IF analysis of NIH/3T3 cells using GTX55812 SUMO1 antibody.
Blue : DAPI
Dilution : 1:100
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REFERENCE
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REVIEW
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