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SUMO2 + SUMO3 antibody [3H12]

Anti-SUMO2 + SUMO3 antibody [3H12] used in IHC (Frozen sections) (IHC-Fr). GTX00699
Anti-SUMO2 + SUMO3 antibody [3H12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00699
Anti-SUMO2 + SUMO3 antibody [3H12] used in Western Blot (WB). GTX00699
Anti-SUMO2 + SUMO3 antibody [3H12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00699
Anti-SUMO2 + SUMO3 antibody [3H12] used in Western Blot (WB). GTX00699

Cat No. GTX00699

Host

Rat

Clonality

Monoclonal

Clone Name

3H12

Isotype

IgG2a

Application

WB, ICC/IF, IHC-Fr, ELISA

Reactivity

Human, Mouse, Rat, Hamster, Simian
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:100-1:500
IHC-Fr 1:100-1:500
ELISA Assay dependent
Not tested in other applications.

Specificity/Sensitivity

This antibody recognizes both SUMO2 and SUMO3, but not SUMO1.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant GST-fused human SUMO3 (full length)

Purification

Purified IgG
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

small ubiquitin like modifier 3 , small ubiquitin like modifier 2 , SMT3A , SMT3H1 , SUMO-3 , Smt3B , HSMT3 , SMT3B , SMT3H2 , SUMO3 , Smt3A

Cellular Localization

Nucleus,Cytoplasm

Database

Research Area

DATA IMAGES

Anti-SUMO2 + SUMO3 antibody [3H12] used in IHC (Frozen sections) (IHC-Fr). GTX00699

GTX00699 IHC-Fr Image

IHC-P analysis of mouse testis tissue section (C57BL/6) using GTX00716 Haploid sperm cell-specific antigen antibody [TRA54].
Fixation : Bouin's solution

Anti-SUMO2 + SUMO3 antibody [3H12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00699

GTX00699 ICC/IF Image

ICC/IF analysis of mouse primary neural progenitor cells using GTX00699 SUMO2 + SUMO3 antibody [3H12].

Anti-SUMO2 + SUMO3 antibody [3H12] used in Western Blot (WB). GTX00699

GTX00699 WB Image

WB analysis of HeLa whole cell lysate using GTX00699 SUMO2 + SUMO3 antibody [3H12].
High molecular multiple bands were observed.

Anti-SUMO2 + SUMO3 antibody [3H12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00699

GTX00699 ICC/IF Image

IHC-P analysis of mouse testis tissue section (8-month) using GTX00716 Haploid sperm cell-specific antigen antibody [TRA54]. Anti-GATA antibody stains nuclei of Sertoli cells in seminiferous tubule while anti-TRA54 antibody stains reproductive cells.
Dilution : 1:50
Antigen retrieval : 10 mM citrate buffer (pH 6.0) for 15min
Fixation : 4% PFA

Anti-SUMO2 + SUMO3 antibody [3H12] used in Western Blot (WB). GTX00699

GTX00699 WB Image

WB analysis of various samples using GTX00699 SUMO2 + SUMO3 antibody [3H12].
Arrow indicates unconjugated SUMO-2/3 proteins. SUMO-2/3 proteins conjugate numerous proteins in vivo, and SUMOylation states vary depending on the kinds of cells and physiological states of them.
Lane 1 : MCF-7 whole cell lysate
Lane 2 : NIH3T3 whole cell lysate
Lane 3 : CHO whole cell lysate
Dilution : 1:1000
SDS-PAGE : 10-20% gradient gel

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 319