Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX102190 diluted at 1:1000

Sample (30 μg of whole cell lysate)
A: adult zebrafish
15% SDS PAGE
GTX102190 diluted at 1:500

Various whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SUMO2 + SUMO3 antibody (GTX102190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.