APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain
Predict Reactivity
Rat, Bovine, Dog, Chicken, Pig, Japanese Medaka, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.56 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SUMO2 + SUMO3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2038069
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
HSMT3 antibody , MGC117191 antibody , SMT3B antibody , SMT3H2 antibody , SUMO3 antibody , SUMO2 antibody , ubiquitin-like protein SMT3A antibody , SMT3 homolog 2 antibody , sentrin 2 antibody , small ubiquitin-related modifier 2 antibody , small ubiquitin-like modifier 2 antibody , ubiquitin-like protein SMT3B antibody , SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) antibody
Cellular Localization
Nucleus
Background
This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
Research Area
DATA IMAGES
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GTX102190 WB Image
Various whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SUMO2 + SUMO3 antibody (GTX102190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX102190 WB Image
Sample (30 μg of whole cell lysate)
A: adult zebrafish
15% SDS PAGE
GTX102190 diluted at 1:500
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GTX102190 WB Image
Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX102190 diluted at 1:1000
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REFERENCE
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REVIEW
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