GeneTex
United States (US)

SUMO2 + SUMO3 (cleaved Gly93) antibody

SUMO2-3-cleaved-Gly93-antibody-GTX86908-WB-1_18121411_962.jpg

Cat No. GTX86908

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:20000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

11 kDa. ( Note )

Specificity/Sensitivity

SUMO2/3 (Cleaved-Gly93) Antibody detects endogenous levels of fragment of activated SUMO2/3 resulting from cleavage adjacent to Gly93.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human SUMO2/3.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC117191, SUMO2/3, SMT3H2, HSMT3, SMT3B, SUMO3, SUMO2/3 (cleaved G93), SUMO2/3 (cleaved Gly93), SUMO2/3 cleaved G93, SUMO2/3 cleaved Gly93, Cleaved SUMO2/3 (G93), Cleaved SUMO2/3 (Gly93)

Cellular Localization

Nuclear bodies.

Background

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins.
DATA IMAGES
SUMO2-3-cleaved-Gly93-antibody-GTX86908-WB-1_18121411_962.jpg

GTX86908 WB Image

Western blot analysis of extracts from HeLa cells, using SUMO2/3 (Cleaved-Gly93) Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339