SUMO2 antibody [AT10F1]

Cell lysates of HeLa (35ug) and recombinant proteins (10ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an EC

Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human SUMO2/3 antibody (1:500) with Texas Red (Red).

Cell lysates of HeLa (35ug) and recombinant proteins (10ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 (1:500, GTX50000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human SUMO2/3 antibody (1:50, GTX50000) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).