GeneTex
  • Country / Location Selection

United States (US)

SUMO2 antibody [AT10F1]

Anti-SUMO2 antibody [AT10F1] used in IHC (Paraffin sections) (IHC-P). GTX50000
Anti-SUMO2 antibody [AT10F1] used in Western Blot (WB). GTX50000
Anti-SUMO2 antibody [AT10F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50000
Anti-SUMO2 antibody [AT10F1] used in Western Blot (WB). GTX50000

Cat No. GTX50000

Host

Mouse

Clonality

Monoclonal

Clone Name

AT10F1

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
FACS Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human SUMO2 (1-93aa) purified from E.coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Small Ubiquitin-Like Modifier 2 , Hsmt3 , Smt3B , Smt3H2 , Sumo3 , Smt3A , Sumo2

Cellular Localization

Nuclear bodies.

Background

SUMO (small ubiquitin-related modifier) is the best-characterized member of a growing family of ubiquitin-related proteins. 8–11 kDa that covalently modify various intracellular proteins. It resembles ubiquitin in its structure, its ability to be ligated to other proteins. SUMO regulates cellular function of a variety of target proteins. SUMO proteins are expressed as their precursor forms. Cleavage of the residues after the ‘GG’ region of these precursors by SUMO-specific proteases in maturation is a prerequisite for subsequent sumoylation. Notably, SUMO2 and SUMO3 precursors have 96% sequence identity, and recent studies have shown protein substrates conjugated with SUMO2 or SUMO3 have similar, if not identical, biological consequences.

Database

Research Area

DATA IMAGES

Anti-SUMO2 antibody [AT10F1] used in IHC (Paraffin sections) (IHC-P). GTX50000

GTX50000 IHC-P Image

Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human SUMO2/3 antibody (1:50, GTX50000) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Anti-SUMO2 antibody [AT10F1] used in Western Blot (WB). GTX50000

GTX50000 WB Image

Cell lysates of HeLa (35ug) and recombinant proteins (10ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an EC

Anti-SUMO2 antibody [AT10F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50000

GTX50000 ICC/IF Image

Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human SUMO2/3 antibody (1:500) with Texas Red (Red).

Anti-SUMO2 antibody [AT10F1] used in Western Blot (WB). GTX50000

GTX50000 WB Image

Cell lysates of HeLa (35ug) and recombinant proteins (10ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 (1:500, GTX50000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

There are currently no references for SUMO2 antibody [AT10F1] (GTX50000). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SUMO2 antibody [AT10F1] (GTX50000). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf