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SUMO2 antibody

Anti-SUMO2 antibody used in Western Blot (WB). GTX54705
Anti-SUMO2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54705

Cat. No. GTX54705

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all SUMO2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human SUMO2 (NP_008868.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887875

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

small ubiquitin like modifier 2 , HSMT3 , SMT3B , SMT3H2 , SUMO3 , Smt3A

Cellular Localization

Nucleus

Background

This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SUMO2 antibody used in Western Blot (WB). GTX54705

GTX54705 WB Image

WB analysis of various sample lysates using GTX54705 SUMO2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-SUMO2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54705

GTX54705 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54705 SUMO2 antibody.
Blue : DAPI

REFERENCE

Application Reference

Zhang J et al. Front Immunol 2019; 10 : 2358 Switch of NAD Salvage to de novo Biosynthesis Sustains SIRT1-RelB-Dependent Inflammatory Tolerance.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399