APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:1000 |
| 1:50-1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.1% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide, corresponding to amino acids 60-110 of Human SUMO-2.
Purification
Purified by antigen-affinity chromatography
From serum
Purity
> 95% (by SDS-PAGE)
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
small ubiquitin like modifier 2 , HSMT3 , SMT3B , SMT3H2 , SUMO3 , Smt3A
Cellular Localization
Nucleus
Background
This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX66829 WB Image
WB analysis of various samples using GTX66829 SUMO2 antibody.
Lane1 : Hela whole cell lysate(40ug)
Lane2 : NIH-3T3 whole cell lysate(40ug)
Lane3 : PC12 whole cell lysate(40ug)
Lane4 : HEK293T whole cell lysate(40ug)
Dilution : 1:500
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GTX66829 IP Image
IP analysis of PC12 cell lysate using GTX66829 SUMO2 antibody (Lane 2 and 3) and nonspecific IgG control antibody (Lane 4 and 5) compared to the input control (Lane 1).
Upward arrow : supernatant
Downward arrow : Immunoprecipitants
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GTX66829 WB Image
WB analysis of various samples using GTX66829 SUMO2 antibody.
Lane1 : Hela whole cell lysate(40ug)
Lane2 : HEK293T whole cell lysate(40ug)
Lane3 : PC12 whole cell lysate(40ug)
Lane4 : CT-26 whole cell lysate(40ug)
Dilution : 1:500
|
REFERENCE
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REVIEW
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