GeneTex

  • Country / Location Selection

United States (US)

SUN1 antibody [HL1947]

Recombinant antibody KO/KD Orthogonal
Anti-SUN1 antibody [HL1947] used in Western Blot (WB). GTX637784
Anti-SUN1 antibody [HL1947] used in IHC (Paraffin sections) (IHC-P). GTX637784
Anti-SUN1 antibody [HL1947] used in Western Blot (WB). GTX637784
Anti-SUN1 antibody [HL1947] used in Western Blot (WB). GTX637784
Anti-SUN1 antibody [HL1947] used in Western Blot (WB). GTX637784
Anti-SUN1 antibody [HL1947] used in Western Blot (WB). GTX637784
Anti-SUN1 antibody [HL1947] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637784

Cat. No. GTX637784

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1947

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SUN1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human SUN1

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Sad1 and UNC84 domain containing 1 , UNC84A

Cellular Localization

Nucleus inner membrane

Background

This gene is a member of the unc-84 homolog family and encodes a nuclear nuclear envelope protein with an Unc84 (SUN) domain. The protein is involved in nuclear anchorage and migration. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2010]

Database

Research Area

DATA IMAGES

Anti-SUN1 antibody [HL1947] used in Western Blot (WB). GTX637784

GTX637784 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (GTX637784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SUN1 antibody [HL1947] used in IHC (Paraffin sections) (IHC-P). GTX637784

GTX637784 IHC-P Image

SUN1 antibody [HL1947] detects SUN1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SUN1 stained by SUN1 antibody [HL1947] (GTX637784) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SUN1 antibody [HL1947] used in Western Blot (WB). GTX637784

GTX637784 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (GTX637784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Multiple bands were observed 80, 90kDa, and 100 kDa. It is possibly due to alternative splicing.
Reference: PMID: 31686651

Anti-SUN1 antibody [HL1947] used in Western Blot (WB). GTX637784

GTX637784 WB Image

Various whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (GTX637784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Multiple bands were observed 80, 90kDa, and 100 kDa. It is possibly due to alternative splicing.
Reference: PMID: 31686651

Anti-SUN1 antibody [HL1947] used in Western Blot (WB). GTX637784

GTX637784 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (GTX637784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-SUN1 antibody [HL1947] used in Western Blot (WB). GTX637784

GTX637784 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (GTX637784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-SUN1 antibody [HL1947] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637784

GTX637784 ICC/IF Image

SUN1 antibody [HL1947] detects SUN1 protein at nuclear envelope by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SUN1 stained by SUN1 antibody [HL1947] (GTX637784) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

There are currently no references for SUN1 antibody [HL1947] (GTX637784). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for SUN1 antibody [HL1947] (GTX637784). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
$ 169
Custom Conjugations