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SUPV3L1 antibody [C2C3], C-term

KO/KD
Anti-SUPV3L1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123034
Anti-SUPV3L1 antibody [C2C3], C-term used in Western Blot (WB). GTX123034
Anti-SUPV3L1 antibody [C2C3], C-term used in Western Blot (WB). GTX123034

Cat. No. GTX123034

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SUPV3L1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SUPV3L1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164944

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Suv3 like RNA helicase , SUV3

Cellular Localization

Mitochondrion matrix , Nucleus

Background

ATPase and DNA/RNA helicase able to unwind DNA/DNA, DNA/RNA and RNA/RNA duplexes in the 5'-3' direction. May protect cells from apoptosis.

Database

Research Area

DATA IMAGES

Anti-SUPV3L1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123034

GTX123034 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using SUPV3L1(GTX123034) antibody (Green) at 1:500 dilution.

Anti-SUPV3L1 antibody [C2C3], C-term used in Western Blot (WB). GTX123034

GTX123034 WB Image

KO/KD

SUPV3L1 antibody [C2C3], C-term detects SUPV3L1 protein by Western blot analysis.
Upper panel: SUPV3L1 antibody [C2C3], C-term (GTX123034)
Lower panel: beta Actin antibody (GTX109639)
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg whole cell lysate/extract of SUPV3L1 shRNA-transfected HeLa cells
7.5 % SDS-PAGE
SUPV3L1 antibody [C2C3], C-term (GTX123034) dilution: 1:1000
beta Actin antibody (GTX109639) dilution: 1:10000

Anti-SUPV3L1 antibody [C2C3], C-term used in Western Blot (WB). GTX123034

GTX123034 WB Image

Sample (30 ug of whole cell lysate)
A: PC-3
7.5% SDS PAGE
GTX123034 diluted at 1:1000

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169