GeneTex
United States (US)

SUPV3L1 antibody [C2C3], C-term

KO/KD
GTX123034_40464_WB_shRNA_watermark_18121410_700.jpg
GTX123034_40646_IFA_18121410_152.jpg
GTX123034_40646_WB_18121410_580.jpg

Cat No. GTX123034

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

PC-3 , HeLa

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SUPV3L1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Suv3 Like Rna Helicase,Suv3,Supv3L1

Cellular Localization

Mitochondrion matrix , Nucleus

Background

ATPase and DNA/RNA helicase able to unwind DNA/DNA, DNA/RNA and RNA/RNA duplexes in the 5'-3' direction. May protect cells from apoptosis.

Database

Research Area

DATA IMAGES
GTX123034_40464_WB_shRNA_watermark_18121410_700.jpg

GTX123034 WB Image

KO/KD

SUPV3L1 antibody [C2C3], C-term detects SUPV3L1 protein by Western blot analysis.
Upper panel: SUPV3L1 antibody [C2C3], C-term (GTX123034)
Lower panel: beta Actin antibody (GTX109639)
A. 30 µg HeLa whole cell lysate/extract
B. 30 µg whole cell lysate/extract of SUPV3L1 shRNA-transfected HeLa cells
7.5 % SDS-PAGE
SUPV3L1 antibody [C2C3], C-term (GTX123034) dilution: 1:1000
beta Actin antibody (GTX109639) dilution: 1:10000

GTX123034_40646_IFA_18121410_152.jpg

GTX123034 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using SUPV3L1(GTX123034) antibody (Green) at 1:500 dilution.

GTX123034_40646_WB_18121410_580.jpg

GTX123034 WB Image

Sample (30 ug of whole cell lysate)
A: PC-3
7.5% SDS PAGE
GTX123034 diluted at 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169