GeneTex
United States (US)

SUV39H1 antibody [44.1]

Cat No. GTX12405

Host Mouse
Clonality Monoclonal
Clone Name 44.1
Isotype IgG1
Application WB, IP, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2-4 μg/ml
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa nuclear extract

Specificity/Sensitivity

Monoclonal Anti-SUV39H1 Histone Methyltransferase recognizes an epitope in the N-terminal (195 amino acids) of human and mouse SUV39H1 Histone Methyltransferase.
PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

recombinant fusion protein MBP-SUV39H1

Purification

Purified immunoglobulin

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Suppressor Of Variegation 3-9 Homolog 1,H3-K9-Hmtase 1,Kmt1A,Mg44,Suv39H,Suv39H1

Background

In eukaryotic cells the histone methylase SUV39H1 and the methyl-lys binding protein HP1 interact to repress transcription at heterochromatic sites. Lys 9 of histone H3 is methylated by SUV39H1, creating a binding site for the chromo domain of HP1. The Lys methylase activity of SUV39H1 resides in the SET domain.SUV39H1 and HP1 are both involved in the repressive functions of the retinoblastoma (Rb) protein. SUV39H1 cooperates with Rb to repress the cyclin E promoter, and in fibrobroblasts that are disrupted for SUV39, the activity of the cyclin E and cyclin A2 genes are specifcally elevated. Chromatin IPs (ChIPs) show that Rb is necessary to direct methylation of histone H3, and is necessary for binding of HP1 to the cyclin E promoter. The SUV39H1-HP1 complex is thus not only involved in heterochromatic silencing but also has a role in repression of euchromatic genes by Rb and perhaps other co-repressor proteins.

Database

Research Area

Package List Price ($)
$ 289