APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
2-4 μg/ml |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HeLa nuclear extract
Product Note
Monoclonal Anti-SUV39H1 Histone Methyltransferase recognizes an epitope in the N-terminal (195 amino acids) of human and mouse SUV39H1 Histone Methyltransferase.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
~2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
recombinant fusion protein MBP-SUV39H1
Purification
Purified immunoglobulin
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
suppressor of variegation 3-9 homolog 1 , H3-K9-HMTase 1 , KMT1A , MG44 , SUV39H
Cellular Localization
Nucleus,nucleoplasm
Background
In eukaryotic cells the histone methylase SUV39H1 and the methyl-lys binding protein HP1 interact to repress transcription at heterochromatic sites. Lys 9 of histone H3 is methylated by SUV39H1, creating a binding site for the chromo domain of HP1. The Lys methylase activity of SUV39H1 resides in the SET domain.SUV39H1 and HP1 are both involved in the repressive functions of the retinoblastoma (Rb) protein. SUV39H1 cooperates with Rb to repress the cyclin E promoter, and in fibrobroblasts that are disrupted for SUV39, the activity of the cyclin E and cyclin A2 genes are specifcally elevated. Chromatin IPs (ChIPs) show that Rb is necessary to direct methylation of histone H3, and is necessary for binding of HP1 to the cyclin E promoter. The SUV39H1-HP1 complex is thus not only involved in heterochromatic silencing but also has a role in repression of euchromatic genes by Rb and perhaps other co-repressor proteins.
Database
Research Area
REFERENCE
There are currently no references for SUV39H1 antibody [44.1] (GTX12405). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SUV39H1 antibody [44.1] (GTX12405). Be the first to share your experience with this product.