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SUV39H1 antibody [N3C3]

Anti-SUV39H1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112263
Anti-SUV39H1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112263
Anti-SUV39H1 antibody [N3C3] used in Western Blot (WB). GTX112263

Cat No. GTX112263

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Monkey
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , H1299 , Raji , Mouse brain

Predict Reactivity

Rat, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SUV39H1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

suppressor of variegation 3-9 homolog 1,H3-K9-HMTase 1,KMT1A,MG44,SUV39H

Cellular Localization

Nucleus , Centromere

Background

This gene is a member of the suppressor of variegation 3-9 homolog family and encodes a protein with a chromodomain and a C-terminal SET domain. This nuclear protein moves to the centromeres during mitosis and functions as a histone methyltransferase, methylating Lys-9 of histone H3. Overall, it plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-SUV39H1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112263

GTX112263 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using SUV39H1(GTX112263) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SUV39H1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112263

GTX112263 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SUV39H1(GTX112263) antibody at 1:200 dilution.

Anti-SUV39H1 antibody [N3C3] used in Western Blot (WB). GTX112263

GTX112263 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SUV39H1 antibody [N3C3] (GTX112263) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169