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SUZ12 antibody

Anti-SUZ12 antibody used in Immunoprecipitation (IP). GTX130179
Anti-SUZ12 antibody used in Western Blot (WB). GTX130179
Anti-SUZ12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130179

Cat. No. GTX130179

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SUZ12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

293T , HeLa , HepG2

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SUZ12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886199

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SUZ12 polycomb repressive complex 2 subunit , CHET9 , JJAZ1

Cellular Localization

Nucleus

Background

This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. The specific function of this gene has not yet been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SUZ12 antibody used in Immunoprecipitation (IP). GTX130179

GTX130179 IP Image

Immunoprecipitation of SUZ12 protein from 293T whole cell extracts using 5 μg of SUZ12 antibody (GTX130179).
Western blot analysis was performed using SUZ12 antibody (GTX130179) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SUZ12 antibody used in Western Blot (WB). GTX130179

GTX130179 WB Image

SUZ12 antibody detects SUZ12 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUZ12 antibody (GTX130179) diluted at a dilution of 1:1000.

Anti-SUZ12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130179

GTX130179 ICC/IF Image

SUZ12 antibody detects SUZ12 protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SUZ12 stained by SUZ12 antibody (GTX130179) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169