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SV40 Large T antigen antibody

Orthogonal
Anti-SV40 Large T antigen antibody used in Western Blot (WB). GTX134377

Cat. No. GTX134377

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Simian virus 40
Package
100 μl ($399),
25 μl ($169)
See all SV40 T-antigen products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Positive Control

293T

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Simian virus 40

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of SV40 Large T antigen (SV40 virus (strain 776)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887263

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Simian virus 40 T antigen ( Large T) , SV40 virus T antigen ( Large T)

Research Area

DATA IMAGES

Anti-SV40 Large T antigen antibody used in Western Blot (WB). GTX134377

GTX134377 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SV40 Large T antibody (GTX134377) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Jennifer A. Hurcombe et al. FASEB BioAdvances 2019; (Epub) Podocyte GSK3α is important for autophagy and its loss detrimental for glomerular function.
Application : WB
Hurcombe JA et al. Nat Commun 2019; 10 (1) : 403 Podocyte GSK3 is an evolutionarily conserved critical regulator of kidney function.
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169