GeneTex
United States (US)

SV40 T-antigen antibody [PAb416]

Cat No. GTX16879

Host Mouse
Clonality Monoclonal
Clone Name PAb416
Isotype IgG2a
Application WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity Simian virus 40
APPLICATION

Application Note

For IF: Use at a concentration of 1 - 5 μg/ml. For IP: Use at a concentration of 1 - 5 μg/ml. For WB: Use at a concentration of 1 - 5 μg/ml. Predicted molecular weight: 82 kDa and 20 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Positive Control

SV80 or SV-T2 cells

Specificity/Sensitivity

GTX16879 recognizes the 94 kDa SV40 large T antigen and the 20.5 kDa SV40 small T antigen.
PROPERTIES

Form

Liquid

Buffer

50mM Sodium phosphate, 0.2% gelatin with 0.1% sodium azide as preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length protein (purified) (SV40).

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Nuclear

Background

SV40 T antigen is encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.
REFERENCE
Package List Price ($)
$ 319