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SV40 T-antigen antibody [PAb416]

Cat. No. GTX16879

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 PAb416

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

 Simian virus 40
Package
500 μl ($399)
See all SV40 T-antigen products

APPLICATION

Application Note

For IF: Use at a concentration of 1 - 5 μg/ml. For IP: Use at a concentration of 1 - 5 μg/ml. For WB: Use at a concentration of 1 - 5 μg/ml. Predicted molecular weight: 82 kDa and 20 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Positive Control

SV80 or SV-T2 cells

Product Note

GTX16879 recognizes the 94 kDa SV40 large T antigen and the 20.5 kDa SV40 small T antigen.

PROPERTIES

Form

Liquid

Buffer

50mM Sodium Phosphate, 0.2% Gelatin

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length protein (purified) (SV40).

Purification

Affinity purified with antigen

Conjugation

Unconjugated

RRID

AB_422449

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Simian virus 40 T antigen ( Large T) , SV40 virus T antigen ( Large T)

Cellular Localization

Nuclear

Background

SV40 T antigen is encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.

Research Area

REFERENCE

Application Reference

Yang CC et al. Cell Rep 2022; 38 (10) : 110472 CRM1-spike-mediated nuclear export of hepatitis B virus encapsidated viral RNA.
Jeffers-Francis LK et al. Antiviral Res 2015; 118 : 46-55 Effect of Leflunomide, * and * on replication of BKPyV in a salivary gland in vitro culture system.
Application : WB
Burger-Calderon R et al. J Virol 2014; 88 (1) : 559-73 Replication of oral BK virus in human salivary gland cells.
Application : WB
Wang J et al. Arch Toxicol 2014; 89 (11) : 2015-25 Sterigmatocystin induces G1 arrest in primary human esophageal epithelial cells but induces G2 arrest in immortalized cells: key mechanistic differences in these two models.
Application : WB
Jeffers LK et al. J Cancer Ther 2013; 4 (3) Correlation of transcription of MALAT-1, a novel noncoding RNA, with deregulated expression of tumor suppressor p53 in small DNA tumor virus models.
Application : WB, IHC-P, IHC-Fr
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399