For IF: Use at a concentration of 1 - 5 μg/ml. For IP: Use at a concentration of 1 - 5 μg/ml. For WB: Use at a concentration of 1 - 5 μg/ml. Predicted molecular weight: 82 kDa and 20 kDa. Optimal dilutions/concentrations should be determined by the researcher.
SV80 or SV-T2 cells
GTX16879 recognizes the 94 kDa SV40 large T antigen and the 20.5 kDa SV40 small T antigen.
50mM Sodium phosphate, 0.2% gelatin with 0.1% sodium azide as preservative
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Full length protein (purified) (SV40).
Affinity purified with antigen
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
SV40 T antigen is encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.