SYPL2 antibody

Cat. No. GTX85272

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, ELISA
Reactivity	Human, Mouse, Rat
Package	100 μg ($409)

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Datasheet
Document

View product citations for antibody GTX85272 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1 μg/mL
ICC/IF	Assay dependent
IHC-P	2.5 μg/mL
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

Human Spleen Tissue Lysate(GTX27921) , Human Spleen Tissue Slide

Product Note

At least two isoforms of SYPL2 are known to exist. SYPL2 antibody will not cross-react with SYPL1.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SYPL2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SYPL.The immunogen is located within the last 50 amino acids of SYPL2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synaptophysin like 2 , MG29

Cellular Localization

Membrane

Background

SYPL2, also known as Mitsugumin 29, was initially identified as a transmembrane protein from the triad junction in skeletal muscle that had significant homology with members of the synaptophysin family. SYPL2 is thought to participate in the excitation-contraction coupling process of skeletal muscle as SYPL2- mice showed reduced muscle contractile force and altered triad junction structure and increased susceptibility to fatigue of the skeletal muscle. SYPL2 plays a critical role in muscle Ca2+ signaling by regulating the process of store-operated Ca2+ entry and interacts with ryanodine receptor (RyR), thereby influencing intracellular Ca2+ homeostasis through changes in the RyR/Ca2+ release function. Co-expression of SYPL2 and RyR in cultured cells leads to apoptotic cell death resulting from the depletion of Ca2+ from the intracellular stores. At least two isoforms of SYPL2 are known to exist. SYPL2 antibody will not cross-react with SYPL1.

Database

Gene ID: 284612 SYPL2
UniProt: Q5VXT5 SYPL2

Research Area

DATA IMAGES

GTX85272 ICC/IF Image

ICC/IF analysis of human spleen cells using GTX85272 SYPL2 antibody.
Working concentration : 20 μg/ml

GTX85272 WB Image

WB analysis of human spleen tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX85272 SYPL2 antibody.
Working concentration : 1 μg/ml

GTX85272 IHC-P Image

IHC-P analysis of human spleen tissue using GTX85272 SYPL2 antibody.
Working concentration : 2.5 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 409

Qty