Salmonella enteritidis LPS antibody [se-1]

Immunochromatography with GTX00861 Salmonella enteritidis LPS antibody [se-1]. Goat anti-S.Enteritidis serum and rabbit anti-Campylobacter jejuni serum were coated onto a specific upper area and under area, respectively, of the same nitrocellulose membrane, while goat anti-mouse IgG was coated onto another specific area (control line) on the same membrane. GTX00861 or C. jejuni MAb conjugated with colloidal gold were mixed with extract of each food poisoning bacteria in well. The strips were soaked and reacted with the mixture. Specific reactivity was shown in the well containing S. Enteritideis (left strip) and C. jujeni (right strip).

WB analysis of various samples using GTX00861 Salmonella enteritidis LPS antibody [se-1]. They showed similar band patterns characteristic of LPS, O-chain repeating unit. MAb (se-01) recognizes common antigenic determinants that are found in the structural components of Salmonella LPS.
Lane 1 : Crude extract of S. Enteritidis cells
Lane 2 : Purified LPS of S.Enteritidis (1 μg)
Dilution : 1:250

WB analysis of several food poisoning bacteria using GTX00861 Salmonella enteritidis LPS antibody [se-1]. This antibody reacts only with S. Enteritidis.
Lane 1 : S. Enteritidis
Lane 2 : Vibrio parahaemolyticus
Lane 3 : Escherichia coli
Lane 4 : E. coli O157:H7

ELISA analysis of crude extract of S. Enteritidis using GTX00861 Salmonella enteritidis LPS antibody [se-1].
Protein coating amount : 100μl (1 μg/ml) per well
Antibody amount : 100μl at indicated dilution

Reactivity of GTX00861 Salmonella enteritidis LPS antibody [se-1] with various food poisoning bacteria.

ELISA analysis of crude extract of S. Enteritidis using GTX00861 Salmonella enteritidis LPS antibody [se-1].
Protein coating amount : 100μl per well at indicated amount
Antibody amount : 100μl (1:500) per well

Extracts of each strain of food poisoning bacteria were coated onto 5 area of a nitrocellulose membrane and reacted with GTX00861 Salmonella enteritidis LPS antibody [se-1].
GTX00861 recognizes LPS of S. Enteritidis, but does not react with any non-Salmonella bacteria tested.
SE: S. Enteritidis
EC: E. coli
CJ: Campylobacter jejuni
VP: V. parahaemolyticus.