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Sec8 antibody

Anti-Sec8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117816
Anti-Sec8 antibody used in IHC (Paraffin sections) (IHC-P). GTX117816

Cat. No. GTX117816

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Sec8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Sec8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618848

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

exocyst complex component 4 , SEC8 , SEC8L1 , Sec8p

Background

The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Sec8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117816

GTX117816 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using Sec8(GTX117816) antibody at 1:200 dilution.

Anti-Sec8 antibody used in IHC (Paraffin sections) (IHC-P). GTX117816

GTX117816 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Sec8(GTX117816) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for Sec8 antibody (GTX117816). Be the first to share your publications with this product.
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REVIEW

Sec8 antibody Cat. No. GTX117816

Rating
( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-Sec8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117816
Date : Anonymous submitted on 22-Sep-2014
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HeLa cells
Blocking : 3% BSA+0.3M Glycine in PBST, 25°C, 0.5Hr
Fixation : methanol/aetone
Permeabilization /Antigen retrieval : 0.2% TritonX-100
Primary Antibody : 1:200, 4°C, 16Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169