Securin antibody immunoprecipitates Securin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of Securin protein by 2 μg of Securin antibody (GTX111938) 12% SDS-PAGE The immunoprecipitated Securin protein was detected by Securin antibody (GTX111938) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Sample (30 ug of whole cell lysate)
A: IMR32
12% SDS PAGE
GTX111938 diluted at 1:1000

Securin antibody detects PTTG1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PTTG1 protein stained by Securin antibody (GTX111938) diluted at 1:500.
Blue: Hoechst 33342 staining.

Securin antibody detects PTTG1 protein at on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
Securin antibody (GTX111938) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min