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Securin antibody

Anti-Securin antibody used in Immunoprecipitation (IP). GTX111938
Anti-Securin antibody used in IHC (Paraffin sections) (IHC-P). GTX111938
Anti-Securin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111938
Anti-Securin antibody used in Western Blot (WB). GTX111938

Cat. No. GTX111938

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Securin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

IMR32

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Securin Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162308

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pituitary tumor-transforming 1 , EAP1 , HPTTG , PTTG , TUTR1

Cellular Localization

Cytoplasm , Nucleus

Background

The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Securin antibody used in Immunoprecipitation (IP). GTX111938

GTX111938 IP Image

Securin antibody immunoprecipitates Securin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of Securin protein by 2 μg of Securin antibody (GTX111938) 12% SDS-PAGE The immunoprecipitated Securin protein was detected by Securin antibody (GTX111938) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Securin antibody used in IHC (Paraffin sections) (IHC-P). GTX111938

GTX111938 IHC-P Image

Securin antibody detects PTTG1 protein at on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
Securin antibody (GTX111938) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Securin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111938

GTX111938 ICC/IF Image

Securin antibody detects PTTG1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PTTG1 protein stained by Securin antibody (GTX111938) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Securin antibody used in Western Blot (WB). GTX111938

GTX111938 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
12% SDS PAGE
GTX111938 diluted at 1:1000

REFERENCE

Application Reference

Park S et al. Oncol Rep 2024; 52 (4) Pituitary tumor?transforming gene 1 regulates the senescence and apoptosis of oral squamous cell carcinoma in a p21?dependent DNA damage response manner.
S Park et al. Oncol Rep 2024; 52 : 135 Pituitary tumor?transforming gene 1 regulates the senescence and apoptosis of oral squamous cell carcinoma in a p21?dependent DNA damage response manner
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169