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Seladin 1 antibody [N1C2]

Anti-Seladin 1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103712
Anti-Seladin 1 antibody [N1C2] used in Western Blot (WB). GTX103712
Anti-Seladin 1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX103712
Anti-Seladin 1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX103712
Anti-Seladin 1 antibody [N1C2] used in Western Blot (WB). GTX103712

Cat No. GTX103712

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

A431 , H1299 , mouse brain

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Seladin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

24-dehydrocholesterol reductase , DCE , Nbla03646 , SELADIN1 , seladin-1

Cellular Localization

Endoplasmic reticulum membrane , Golgi apparatus membrane

Background

This gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Seladin 1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103712

GTX103712 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Seladin 1(GTX103712) antibody at 1:200 dilution.

Anti-Seladin 1 antibody [N1C2] used in Western Blot (WB). GTX103712

GTX103712 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX103712 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Seladin 1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX103712

GTX103712 IHC-P Image

Seladin 1 antibody [N1C2] detects Seladin 1 protein at cytoplasm on rat adrenal gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat adrenal gland.
Seladin 1 antibody [N1C2] (GTX103712) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Seladin 1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX103712

GTX103712 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Seladin 1(GTX103712) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Seladin 1 antibody [N1C2] used in Western Blot (WB). GTX103712

GTX103712 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
Seladin 1 antibody
GTX103712 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169