Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [N1C2] (GTX103712) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Seladin 1(GTX103712) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Seladin 1(GTX103712) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Seladin 1 antibody [N1C2] detects Seladin 1 protein at cytoplasm on rat adrenal gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat adrenal gland.
Seladin 1 antibody [N1C2] (GTX103712) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min